In vitro fertilization (IVF) and development of mouse embryos
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Published:
Jul 27, 1994
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Abstract
Fl female mice of 3-4 weeks old derived from the CBA/ca x C57BL/6J strain were superovulated with intraperitoneal injections of 5 1.U. of PMSG and HCG 48 hours apart. At 15 hours after HCG, the oocytes were fertilized with caudal epididymal sperm obtained from males of 10-15 weeks old of CBA/ca x C57BL/6J strain. The insemination concentrations were between 1-2 million sperm cells per mL of medium. At five hours after insemination, the oocytes were washed through a few series of medium changes to remove excess sperm and cumulus cells. The fertilized oocytes were cultured in an incubator in humidified 5% C02 in air at 370C. Embryonic development was recorded until the blastocyst stage. Based on 2-cell stage embryos, fertilization rate of 90.7% was obtained and of the proportion of 2-cell stage embryos, 97.6% developed to the blastocyst stage.
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How to Cite
Abdullah, R. B., Ng, S. Y., & Bakar, F. (1994). In vitro fertilization (IVF) and development of mouse embryos. Malaysian Journal of Science, 15(1&2), 19–20. Retrieved from http://ojie.um.edu.my/index.php/MJS/article/view/4740
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